TAC1和PRLR基因在绵羊不同繁殖状态下的表达模式分析

doi:10.11843/j.issn.0366-6964.2018.02.004

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (2): 253-262.doi: 10.11843/j.issn.0366-6964.2018.02.004

TAC1和PRLR基因在绵羊不同繁殖状态下的表达模式分析

李晓雨¹, 贺小云¹, 刘秋月¹, 王翔宇¹, 郭晓飞¹, 夏青¹, 胡文萍¹, 张效生², 张金龙², 储明星^1*, 狄冉^1*

1. 中国农业科学院北京畜牧兽医研究所, 农业部动物遗传育种与繁殖重点实验室, 北京 100193;
2. 天津市畜牧兽医研究所, 天津 300381

收稿日期:2017-08-04 出版日期:2018-02-23 发布日期:2018-02-23
通讯作者: 储明星,博士,研究员,博士生导师,E-mail:mxchu@263.net;狄冉,博士,副研究员,硕士生导师,E-mail:dirangirl@163.com
作者简介:李晓雨(1991-),女,安徽太和人,硕士生,主要从事绵羊优异繁殖性状分子机理研究,E-mail:lixiaoyudongke@163.com;贺小云(1990-),男,山西运城人,硕士生,主要从事绵羊优异繁殖性状分子机理研究,E-mail:hedayun@sina.cn。
基金资助:
国家自然科学基金（31572371）；国家转基因科技重大专项（2016ZX08009-003-006；2016ZX08010-005-003）；国家肉羊产业技术体系专项（CARS-38）；中央级公益性科研院所基本科研业务费专项（Y2017JC24；2017ywf-zd-13；2013ywf-zd-1；2015ywf-zd-2；2015ywf-zd-8）；中国农业科学院科技创新工程（ASTIP-IAS13；CAAS-XTCX2016010-01-03；CAAS-XTCX2016010-03-03；CAAS-XTCX2016011-02-02）；宁夏农林科学院科技创新先导资金项目（DWJLC-2016001）；内蒙古自治区科技重大专项；农业科研杰出人才及其创新团队项目；国家万人计划科技创新领军人才项目

Expression Pattern Analysis of TAC1 and PRLR Genes in Different Reproductive States of Sheep

LI Xiao-yu¹, HE Xiao-yun¹, LIU Qiu-yue¹, WANG Xiang-yu¹, GUO Xiao-fei¹, XIA Qing¹, HU Wen-ping¹, ZHANG Xiao-sheng², ZHANG Jin-long², CHU Ming-xing^1*, DI Ran^1*

1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Tianjin Institute of Animal Sciences, Tianjin 300381, China

Received:2017-08-04 Online:2018-02-23 Published:2018-02-23

摘要/Abstract

摘要：

旨在进一步揭示TAC1和PRLR基因在绵羊季节性发情调控和繁殖时期转换中的作用。本研究利用实时荧光定量PCR技术分析TAC1和PRLR基因在不同光照条件下的成年苏尼特母羊（季节性发情品种）和处于不同繁殖时期的成年小尾寒羊母羊（常年发情品种）10种组织（下丘脑、垂体、松果体、大脑、小脑、卵巢、子宫体、输卵管、肾、肾上腺）中的表达模式。结果表明：1）TAC1和PRLR基因在两品种绵羊10种组织中均有表达，且组织表达特征基本一致，TAC1基因主要表达于性腺轴组织，PRLR基因主要在垂体和肾上腺中表达。2）TAC1和PRLR基因在长光照（LP）条件下苏尼特羊各组织中的表达量均高于短光照（SP）下表达量，在小尾寒羊大多数组织中黄体期表达量高于卵泡期。3）由短光照转换为长光照后，TAC1基因在苏尼特羊垂体中的表达量缓慢增加，在长光照49天时表达量极显著高于短光照和其它长光照时间点（P<0.01）；PRLR基因在苏尼特羊垂体中表达量从LP3D开始显著升高（P<0.05），至LP21D时达到峰值，下丘脑中表达量从LP15D开始显著高于短光照（P<0.05），且有持续升高的趋势。以上结果暗示了TAC1和PRLR基因可能涉及绵羊季节性繁殖和繁殖时期转换的调控，明确了它们发挥作用的主要组织，同时揭示了这2个基因由短光照转换为长光照后的表达变化趋势，发现PRLR基因在垂体和下丘脑中具有不同的响应模式。

Abstract:

The aim of this study was to reveal the regulatory roles of TAC1 and PRLR genes in seasonal estrus and transition between reproductive stages of sheep. In this study, the expression patterns of TAC1 and PRLR genes in 10 tissues (hypothalamus, pituitary, pineal body, brain, cerebellum, ovary, uterus, oviduct, kidney, adrenal gland) of adult Sunite (seasonal estrus breed) ewes under different photoperiods and adult Small Tail Han (STH, year-round estrus breed) ewes at different reproductive stages were analyzed by q-PCR method. The results showed that:1) The expression of TAC1 and PRLR genes were detected in all the 10 tissues of the two sheep breeds, and the expression characteristics were basically similar. TAC1 gene was mainly expressed in gonadal axis tissues, and PRLR gene was mainly expressed in pituitary and adrenal gland. 2) The expression of TAC1 and PRLR genes were higher in Sunite tissues under long photoperiod than those under short photoperiod, and for most tissues of STH sheep, the expression levels of the 2 genes were higher in luteal phase than that in follicular phase. 3) The expression level of TAC1 gene in the pituitary was increased slowly from short photoperiod to long photoperiod, and reached the highest level at LP49D, which was significantly higher than short and other long photoperiod points(P<0.01). The PRLR gene expression level in the pituitary of Sunite sheep was significantly increased from LP3D (P<0.05), reaching peak at LP21D; in hypothalamus, the expression level was significantly higher from LP15D (P<0.05) than that short photoperiod, and there was a tendency to continue to rise. These results indicate that TAC1 and PRLR genes may be involved in the regulation of sheep seasonal reproduction and the transition of reproductive stages, and identify the main tissues where the 2 genes may play important roles. Meanwhile this study reveal the expression trend of the 2 genes from short photoperiod to long photoperiod and different response patterns of PRLR gene in pituitary and the hypothalamus.

中图分类号:

S826.2

李晓雨, 贺小云, 刘秋月, 王翔宇, 郭晓飞, 夏青, 胡文萍, 张效生, 张金龙, 储明星, 狄冉. TAC1和PRLR基因在绵羊不同繁殖状态下的表达模式分析[J]. 畜牧兽医学报, 2018, 49(2): 253-262.

LI Xiao-yu, HE Xiao-yun, LIU Qiu-yue, WANG Xiang-yu, GUO Xiao-fei, XIA Qing, HU Wen-ping, ZHANG Xiao-sheng, ZHANG Jin-long, CHU Ming-xing, DI Ran. Expression Pattern Analysis of TAC1 and PRLR Genes in Different Reproductive States of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 253-262.

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TAC1和PRLR基因在绵羊不同繁殖状态下的表达模式分析

Expression Pattern Analysis of TAC1 and PRLR Genes in Different Reproductive States of Sheep

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